Spontaneous Cell Fusion of Acute Leukemia Cells and Macrophages Observed in Cells with Leukemic Potential

• DOI: 10.1593/neo.12736
• Publication Date: 2015-04-23T13:00:42Z
• Authors: Ines Martin-Padura; Paola Marighetti; Giuliana Gregato; Alice Agliano; Omar Malazzi; Patrizia Mancuso; Giancarlo Pruneri; Andrea Viale; Francesco Bertolini
• Keywords: Cell fusion

Cell fusion plays a well-recognized physiological role during development, while its function progression is still unclear. Here, we show that acute myeloid leukemia (AML) cells spontaneously fused with murine host in vivo. AML most cases mouse macrophages. Other targets of cell were dendritic and endothelial cells. Cytogenetic molecular analysis revealed successive recipients conserved detectable amounts parental DNA. Moreover, AML1-ETO model where female AML1-ETO-leukemic cells, expressing CD45.2, injected congenic CD45.1 male mice found hybrid both allelic types CD45 XXY set sexual chromosomes. More importantly, the protein was transferred When sorted reinjected secondary recipient, they gave rise to 100% penetrance similar time onset leukemic Our data indicate vivo cancer may be mechanism gene transfer for dissemination suggest used identify unrecognized leukemogenic genes are able perpetuate