Determination of Apoptotic Effect of Juglone on Human Bladder Cancer TCC-SUP and RT-4 Cells: An In Vitro Study

• DOI: 10.1615/jenvironpatholtoxicoloncol.2018025226
• Publication Date: 2018-07-10T15:50:10Z
• Authors: Dilek Bayram; İlkay Armagan; Meltem Özgöcmen; Nurgül Senol; Mustafa Calapoglu
• Keywords: Juglone; Bromodeoxyuridine; Terminal deoxynucleotidyl transferase; Spheroid

This study aimed to investigate the effects of juglone on human bladder carcinoma cell lines TCC-SUB and RT-4 in monolayer spheroid cultures. Cells were treated with at 24, 48, 72 h incubation. The activity caspase-3 was detected vitro using a colorimetric assay kit according manufacturer's instructions. bromodeoxyuridine (BrdU) labeling index used determine cells synthesis phase. terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling death both control group had large S-phase fraction many nuclei observed be positive for BrdU. dead count higher applied than controls. We conclude that significantly inhibits proliferation induces apoptosis vitro.