Effects of Boldine on Antioxidants and Allied Inflammatory Markers in Mouse Models of Asthma

• DOI: 10.1615/jenvironpatholtoxicoloncol.2020034039
• Publication Date: 2020-06-12T16:04:16Z
• Authors: Wei Li; Vishnu Priya Veer...; Weining Ma
• Keywords: Male; Aporphines; Ovalbumin; Anti-Inflammatory Agents; Immunoglobulin E; Antioxidants; Asthma; Respiratory Function Tests; 3. Good health; Eosinophils; Mice, Inbred C57BL; Disease Models, Animal; Mice; Animals; Cytokines; Anti-Asthmatic Agents; Reactive Oxygen Species; Bronchoalveolar Lavage Fluid; Lung; Biomarkers

Asthma is marked by chronic irritation in the airway lumen of the lungs due to the accretion of inflammatory cells that influence the regular inhalation process. An extended buildup of inflammation leads to oxidative pressure and the repression of antioxidant functions. In the current study, a potential compound, boldine, was tested for the containment of provocative markers along the path of antiasthmatic activity in an ovalbumin (OVA)-induced asthmatic mice model. As an effect, the boldine (10 and 20 mg/kg) treatment suppressed inflammatory cells such as eosinophil, macrophage, neutrophil, lymphocyte, and other inflammatory markers in the bronchoalveolar lavage fluid (BALF) of OVA-induced mice. Likewise, immunoglobulin E (IgE) levels were drastically condensed in the serum of boldine-treated animals. Levels of enzymatic and nonenzymatic antioxidants, such as superoxide dismutase (SOD) and glutathione (GSH), were upregulated in the boldine treatment group compared to the asthmatic control group, which displays the antioxidant effects of boldine on asthmatic animals. Interestingly, the reactive oxygen species (ROS) and malonaldehyde (MDA) levels were repressed in the BALF of boldine-treated mice groups. Therefore, the effects of boldine are significant for the management of asthma, reducing the accrual of inflammatory cells, along with other inflammatory markers, while improving antioxidant markers and containing ROS. Hence, boldine may be an option for clinical trials of chronic asthma management.