Ginkgo biloba Extract Mechanism Inhibits Hepatocellular Carcinoma through the Nuclear Factor-κB/p53 Signaling Pathway

• DOI: 10.1615/jenvironpatholtoxicoloncol.2020034510
• Publication Date: 2020-06-05T21:07:34Z
• Authors: Ruike Wang; Xiaomei Shao; Jiping Yang; Zhenzhen Liu; Lisah Chew; Ying Shao
• Keywords: Viability assay

Ginkgo biloba extract EGb761 conveys an anticancer effect, but little is known regarding its role in hepatocellular carcinoma (HCC). Our study aims to determine the effect of on HCC cell lines and clarify underlying molecular mechanism. We explore biological functions using morphological observation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cytotoxic analysis. investigate effects proliferation apoptosis cells plate clone formation, proliferating nuclear antigen, terminal deoxynucleotidyl transferase d-untranslated protein nick end labeling assays. Protein expressions NF-κB/p53 signal pathway were detected identified immunohistochemistry. The p53 signaling was further confirmed by adding pifithrin (PFT)-α, inhibitor p53. that inhibits growth, reduces viability, promotes cells. In addition, increases human carcinomas (HepG2) a dose-dependent manner. also find exerts HepG2 activating inhibiting factor (NF)-κB pathways. This confirms triggers through pathway.