SHP1 Decreases Level of P-STAT3 (Ser727) and Inhibits Proliferation and Migration of Pancreatic Cancer Cells

• DOI: 10.1615/jenvironpatholtoxicoloncol.2020035980
• Publication Date: 2020-11-19T15:41:50Z
• Authors: Mingming Zhang; Xiaoru Hu; Ye Kang; Zhe Wang; Wenyang Zhou; Chang Liu; Xianghong Yang
• Keywords: Pancreatic Neoplasms; STAT3 Transcription Factor; 0303 health sciences; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Protein Tyrosine Phosphatase, Non-Receptor Type 6; Humans; Cell Proliferation; 3. Good health

To identify the direct effects and functional mechanisms of SHP1, plus its relationship with STAT3 in pancreatic cancer.Immunohistochemistry Western blot assays were used to test SHP1 expression cancer. The functions cancer cells analyzed by cell viability, colony formation, flow cytometric analysis apoptosis, migration invasion assays. Co-immunoprecipitation, combined shotgun mass spectrometry, verified or indirect interactions JAK1 p-STAT3(Ser727). Non-labeling quantitative proteomics evaluated effect on protein levels. PRM phosphorylation modification confirmed p-STAT3(Ser727) levels.SHP1 was missing weakly expressed human ductal adenocarcinoma tissues cells: PANC-1, AsPC-1, BxPC-3, SW1990. inhibited proliferation migration. had a slight level PANC-1 cells. decreased at 0.53 multiple. Co-IP revealed no between SHP1and complex patterns.These results suggest that negatively regulate progression. It inhibits activation decreasing serine 727.