Measurement of (1-3)-.BETA.-D-Glucan Derived from Different Gauze Types

• DOI: 10.1620/tjem.217.117
• Publication Date: 2009-02-18T05:58:12Z
• Authors: Hajime Kanamori; Keiji Kanemitsu; Tomomitsu Miyasaka; Koken Ameku; Shiro Endo; Tetsuji Aoyagi; Ken Inden; Masumitsu Hatta; Natsuo Yamamoto; Hiroyuki Kunishima; Hisakazu Yano; Koki Kaku; Yoichi Hirakata; Mitsuo Kaku
• Keywords: 0301 basic medicine; 03 medical and health sciences; Time Factors; beta-Glucans; Humans; Occlusive Dressings; 6. Clean water; 3. Good health

(1-3)-β-D-glucan (BDG) is a cell-wall polysaccharide component found in most fungi. The measurement of BDG useful diagnostic marker for invasive fungal infections. However, it well known that interfering substances can result false positive reactions. We encountered patient who underwent lung transplantation and presented with highly elevated values, despite having no evidence infection. therefore hypothesized values were originated from the gauze products used during surgery. While contain BDG, there have been previous reports to quantitatively correlate amount usage levels. In this study, we extracted various measured better understand degree which contributes values. Six types commonly surgical selected our study. Each was immersed sterile, purified water up 120 minutes. At set intervals, extracts measured. Purified samples without as negative controls (< 4 pg/ml). After 120-minute extraction, levels varied greatly depending on products, ranging 11.7 pg/ml 6612 pg/ml. made lyocell, fiber produced wood pulp cellulose, yielded lowest probably would not cause There need development product does contribute