Protection and Differentiation of Infected from Vaccinated Animals by an Inactivated Recombinant Newcastle Disease Virus/Avian Influenza H5 Vaccine

• DOI: 10.1637/8767-033109-resnote.1
• Publication Date: 2010-03-25T14:05:02Z
• Authors: Bernardo Lozano-D...; Ernesto Soto-Priante; David Sarfati-Miz...; Felipa Castro-Per...; Ricardo Flores-Ca...; Elizabeth Loza-Rubio; Manuel Gay-Gutiérrez
• Keywords: Newcastle Disease; Inactivated vaccine; Hemagglutination assay; Attenuated vaccine

Specific-pathogen-free chickens immunized at 14 days of age with either an inactivated recombinant Newcastle disease virus–LaSota/avian influenza H5 (K-rNDV-LS/AI-H5) vaccine or a killed disease/avian whole-virus (K-ND/AI) were protected from when challenged A/chicken/Queretaro/14588-19/95 (H5N2), high pathogenicity avian virus (HPAIV) strain isolated in Mexico 1995, Mexican velogenic viscerotropic (VVNDV) 21 postvaccination. All nonvaccinated HPAIV VVNDV succumbed to disease, while those vaccinated K-rNDV-LS/AI-H5 K-ND/AI severe clinical signs and death. Both vaccines induced hemagglutination-inhibition (HI) antibody responses against NDV AIV. Antibodies AIV nucleoprotein not detected by enzyme-linked immunosorbent assay (ELISA) birds the rNDV-LS/AI-H5 vaccine. These became positive for antibodies ELISA only after challenge HPAIV. The data clearly indicate that confers protection comparable conventional both AIV, still allowing differentiation infected animals HI tests.