ISOLATION, IDENTIFICATION, AND GENOME ANALYSIS OF LUNG PATHOGENIC KLEBSIELLA PNEUMONIAE (LPKP) IN FOREST MUSK DEER

• DOI: 10.1638/2016-0241.1
• Publication Date: 2018-01-03T15:13:12Z
• Authors: Wei Zhao; Qing Tian; Yan Luo; Yin Wang; Ze-xiao Yang; Xue-ping Yao; Jian-guo Cheng; Xin Zhou; Wu-you Wang
• Keywords: Lung Diseases; 0303 health sciences; Deer; Anti-Bacterial Agents; Klebsiella Infections; 3. Good health; Klebsiella pneumoniae; Mice; RNA, Bacterial; 03 medical and health sciences; RNA, Ribosomal, 16S; Drug Resistance, Bacterial; Animals; Phylogeny

Klebsiella pneumoniae is an important pathogen commonly associated with opportunistic infections. In this study, lung pathogenic K. (LPKP) was isolated and identified from suppurative in forest musk deer by conventional methods 16S ribosomal RNA sequence analysis. Median lethal dose histopathologic analysis were used to demonstrate pathogenicity of the organism mice. Furthermore, a draft genome LPKP sequenced, its virulence genes detected. One hundred twenty-two encoded determinant capsule polysaccharide (CPS), lipopolysaccharide, fimbriae, outer membrane proteins, iron acquisition, urease. particular, 20 CPS-related highly conserved LPKP, U, NTUH-KP35, KP-1. All strains as capsular type K54. This first report K54 causing pneumonia animal. The results study provided basis for understanding laid foundation development vaccines disease.