ACTIVITY OF INTRACELLULAR PHOSPHOLIPASE A1AND A2IN GIARDIA LAMBLIA

• DOI: 10.1645/ge-1038r3.1
• Publication Date: 2007-11-15T19:00:49Z
• Authors: Javier Vargas-Vil...; Brenda Leticia Es...; Benito David Mata...; Rebeca Palacios-C...; Elva Cortes-Gutié...; Mario Morales-Val...; Adriana Sampayo-R...; Salvador Said-Fer...
• Keywords: Giardia lamblia; Giardia

Neither phospholipase A1 (PLA A1) nor A2 A2), their respective genes, have been identified in Giardia lamblia, even though they are essential for lipid metabolism this parasite. A method to identify, isolate, and characterize these enzymes is needed. The activities of PLA were analyzed a total extract (TE) vesicular (P30) soluble (S30) subcellular fractions G. lamblia trophozoites; the effects several chemical physicochemical factors on investigated. assays performed using substrate labeled with 14C, mass 14C-product was quantified. activity present TE P30 S30 fractions, it dependent pH concentrations protein Ca2 . In all trophozoite preparations, inhibited by ethylenediaminetetraacetic acid Rosenthal's inhibitor. These results suggest that possesses isoforms may be or associated membranes. addition participating phospholipid metabolism, could play important roles cytopathogenicity