Isolation of Toxoplasma gondii from Animals in Durango, Mexico

• DOI: 10.1645/ge-1874.1
• Publication Date: 2009-05-01T17:18:30Z
• Authors: J. P. Dubey; G. V. Velmurugan; C. Alvarado-Esquivel; D. Alvarado-Esquivel; S. Rodríguez-Peña; S. Martínez-García; A. González-Herrera; L. R. Ferreira; O. C. H. Kwok; C. Su
• Keywords: Isolation; Toxoplasmosis

Little is known concerning the epidemiology of Toxoplasma gondii infection in people and animals rural Mexico. Serum samples tissues from 150 dogs (Canis familaris), cats (Felis catus), 65 opossums (Didelphis virginianus), 249 rats (Rattus spp.), 127 mice (Mus musculus), 69 squirrels (Spermophilus variegatus) Durango area were evaluated for T. infection. Using a modified agglutination test serum dilution 1:25, antibodies to this parasite found 68 (45.3%) dogs, 14 (9.3%) cats, 11 (16.6%) 66 opossums, 2 (0.8%) rats, 4 (3.1%) mice, 0 squirrels. Tissues (brain heart) bioassayed presence gondii. Viable was isolated 3 28 seropositive 5 8 but not other animals. The DNA obtained isolates 6 free-range chickens Mexico, previously isolated, genotyped. PCR-RFLP typing, which used markers (B1, SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico), identified genotypes. One genotype (the chicken isolates) belongs clonal Type III lineage, three genotypes reported previous reports, 1 unique.