The miR-15 Family Enhances the Radiosensitivity of Breast Cancer Cells by Targeting G2Checkpoints

• DOI: 10.1667/rr13784.1
• Publication Date: 2015-01-16T19:40:55Z
• Authors: Zijie Mei; Tingshi Su; Junjie Ye; Chunxu Yang; Shimin Zhang; Conghua Xie
• Keywords: Radiosensitivity; Clonogenic assay; Radioresistance

Enhancing radiosensitivity is an important area of investigation for improving breast cancer therapy outcomes. The aim this study was to assess the role miR-15 family in cells. MicroRNAs (miRNAs) encoded by cluster are known induce G1 arrest and apoptosis targeting checkpoints anti-apoptotic B cell lymphoma 2 (BCL-2) gene. However, effect on G2/M remains poorly understood. In current study, cells transfected with miR-15a/15b/16 mimic or inhibitor were irradiated examined by: clonogenic assays, phosphorylated H2AX assay, flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), real-time PCR Western blot. Real-time also used monitor time-dependent changes expression after irradiation. A putative target site within Chk1 Wee1 3' UTRs confirmed using luciferase reporter assays. Additionally, siRNA validate our we investigated effects radiation found a change postirradiation, as well increase family-mediated sensitization radiation. induced associated persistent unrepaired DNA damage, abrogation radiation-induced G2 suppressed proliferation, appear involve both checkpoint kinase 1 (Chk1) Wee1. addition, that inhibition could not resistance These findings suggest contributes increased influencing proteins.