Mitochondrial Targeting of a Catalase Transgene Product by Plasmid Liposomes Increases RadioresistanceIn VitroandIn Vivo

• DOI: 10.1667/rr1424.1
• Publication Date: 2009-04-28T19:37:34Z
• Authors: Michael W. Epperly; J. A. Melendez; Xichen Zhang; Suhua Nie; Linda Pearce; James Peterson; Darcy Franicola; Tracy Dixon; Benjamin A. Green...; Paavani Komanduri; Hong Wang; Joel S. Greenberger
• Keywords: Radioresistance

Epperly, M. W., Melendez, J. A., Zhang, X., Nie, S, Pearce, L., Peterson, J., Franicola, D., Dixon, T., Greenberger, B. Komanduri, P., Wang, H. and S. Mitochondrial Targeting of a Catalase Transgene Product by Plasmid Liposomes Increases Radioresistance In Vitro Vivo. Radiat. Res. 171, 588-595 (2009).To determine whether increased mitochondrially localized catalase was radioprotective, human transgene cloned into small pSVZeo plasmid to the mitochondria 32D cl 3 cells adding mitochondrial localization sequence MnSOD (mt-catalase). The cell lines 32D-Cat 32D-mt-Cat had biochemical activity as confirmed Western blot analysis compared parent cells. MnSOD-overexpressing line, 2C6, decreased baseline that in 2C6-Cat 2C6-mt-Cat subclonal lines. were more radioresistant than cells, but both relative not 2C6 Intratracheal injection mt-catalase-plasmid liposome complex (mt-Cat-PL) catalase-plasmid (Cat-PL) resistance C57BL/6NHsd female mice 20 Gy thoracic irradiation MnSOD-plasmid liposomes. Thus targeted overexpression is radioprotective vitro vivo.