Genomic Instability in Human Lymphocytes Irradiated with Individual Charged Particles: Involvement of Tumor Necrosis Factor α in Irradiated Cells but not Bystander Cells
Cell Survival
610
Radiation Dosage
Chromosomes
Genomic Instability
name=Radiation
/dk/atira/pure/subjectarea/asjc/2700/2741
03 medical and health sciences
Humans
Lymphocytes
name=Biophysics
Cells, Cultured
Chromosome Aberrations
/dk/atira/pure/subjectarea/asjc/1100/1101
0303 health sciences
/dk/atira/pure/subjectarea/asjc/3100/3108
Genome, Human
Tumor Necrosis Factor-alpha
Dose-Response Relationship, Radiation
Bystander Effect
name=SDG 3 - Good Health and Well-being
3. Good health
name=Agricultural and Biological Sciences (miscellaneous)
name=Radiology Nuclear Medicine and imaging
/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
/dk/atira/pure/subjectarea/asjc/1300/1304
DOI:
10.1667/rr3298
Publication Date:
2006-07-17T20:57:56Z
AUTHORS (9)
ABSTRACT
Exposure to ionizing radiation can increase the risk of cancer, which is often characterized by genomic instability. In environmental exposures to high-LET radiation (e.g. 222Ra), it is unlikely that many cells will be traversed or that any cell will be traversed by more than one alpha particle, resulting in an in vivo bystander situation, potentially involving inflammation. Here primary human lymphocytes were irradiated with precise numbers of 3He2+ ions delivered to defined cell population fractions, to as low as a single cell being traversed, resembling in vivo conditions. Also, we assessed the contribution to genomic instability of the pro-inflammatory cytokine tumor necrosis factor alpha (TNFA). Genomic instability was significantly elevated in irradiated groups (> or = two-fold over controls) and was comparable whether cells were traversed by one or two 3He2+ ions. Interestingly, substantial heterogeneity in genomic instability between experiments was observed when only one cell was traversed. Genomic instability was significantly reduced (60%) in cultures in which all cells were irradiated in the presence of TNFA antibody, but not when fractions were irradiated under the same conditions, suggesting that TNFA may have a role in the initiation of genomic instability in irradiated cells but not bystander cells. These results have implications for low-dose exposure risks and cancer.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (66)
CITATIONS (40)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....