Genomic Instability in Human Lymphocytes Irradiated with Individual Charged Particles: Involvement of Tumor Necrosis Factor α in Irradiated Cells but not Bystander Cells

• DOI: 10.1667/rr3298
• Publication Date: 2006-07-17T20:57:56Z
• Authors: Stephen R. Moore; Sam Marsden; Denise Macdonald; Stephen Mitchell; Melvyn Folkard; Barry Michael; Dudley T. Goodhead; Kevin M. Prise; Munira A. Kadhim
• Keywords: Bystander effect

Moore, S. R., Marsden, S., Macdonald, D., Mitchell, Folkard, M., Michael, B., Goodhead, D. T., Prise, K. M. and Kadhim, A. Genomic Instability in Human Lymphocytes Irradiated with Individual Charged Particles: Involvement of Tumor Necrosis Factor α Cells but not Bystander Cells. Radiat. Res. 163, 183–191 (2005).Exposure to ionizing radiation can increase the risk cancer, which is often characterized by genomic instability. In environmental exposures high-LET (e.g. 222Ra), it unlikely that many cells will be traversed or any cell more than one particle, resulting an vivo bystander situation, potentially involving inflammation. Here primary human lymphocytes were irradiated precise numbers 3He2 ions delivered defined population fractions, as low a single being traversed, resembling conditions. Also, we assessed contribution instability pro-inflammatory cytokine tumor necrosis factor (TNFA). was significantly elevated groups (≥twofold over controls) comparable whether two ions. Interestingly, substantial heterogeneity between experiments observed when only traversed. reduced (60%) cultures all presence TNFA antibody, fractions under same conditions, suggesting may have role initiation cells. These results implications for low-dose exposure risks cancer.