Differential gene expression profiles of invasive and non-invasive non-functioning pituitary adenomas based on microarray analysis

• DOI: 10.1677/erc-10-0018
• Publication Date: 2010-03-04T03:34:50Z
• Authors: Françoise Galland; Ludovic Lacroix; Patrick Saulnier; Philippe Dessen; Geri Meduri; Michèle Bernier; Stéphane Gaillard; Jean Guibourdenche; Thierry Fournier; Danièle Evain-Brion; Jean Michel Bidart; Philippe Chanson
• Keywords: Adenoma; Adult; Aged, 80 and over; Male; 0301 basic medicine; Myosin Heavy Chains; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression Profiling; Myosin Type V; Middle Aged; Microarray Analysis; Immunohistochemistry; Neoplasm Proteins; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; Humans; Female; Pituitary Neoplasms; Neoplasm Recurrence, Local; Insulin-Like Growth Factor Binding Protein 5; Aged

Non-functioning pituitary adenomas (NFPAs) may be locally invasive. Markers of invasiveness are needed to guide patient management and particularly the use adjuvant radiotherapy. To examine whether invasive NFPAs display a specific gene expression profile relative non-invasive tumors, we selected 40 (38 gonadotroph type) classified them as (n=22) or (n=18) on basis magnetic resonance imaging surgical findings. We then performed pangenomic analysis with 44k Agilent human whole genome oligonucleotide microarray in order identify genes differential between NFPAs. Candidate were tested qRT-PCR. Prediction class showed that 346 differed (P<0.001), which 233 up-regulated 113 down-regulated tumors. On Ingenuity networks degree up- down-regulation versus 35 for quantification by Overexpression only four was confirmed, namely IGFBP5 (P=0.02), MYO5A (P=0.04), FLT3 (P=0.01), NFE2L1 (P=0.02). At protein level, myosin 5A (MYO5A) immunostaining stronger than Molecular signature allows differentiate 'grossly' from The product one these genes, MYO5A, useful marker tumor invasiveness.