RET/PTC-induced gene expression in thyroid PCCL3 cells reveals early activation of genes involved in regulation of the immune response

• DOI: 10.1677/erc.1.00947
• Publication Date: 2005-06-09T17:38:50Z
• Authors: E Puxeddu; J A Knauf; M A Sartor; N Mitsutake; E P Smith; M Medvedovic; C R Tomlinson; S Moretti; J A Fagin
• Keywords: JUNB; STAT1

RET/PTC rearrangements represent key genetic events involved in papillary thyroid carcinoma (PTC) initiation. The aim of the present study was to identify early changes gene expression induced by cells. For this purpose, microarray analysis conducted on PCCL3 cells conditionally expressing RET/PTC3 oncogene. Gene profiling 48 h after activation identified a statistically significant modification 270 genes. Quantitative PCR confirmation 20 these demonstrated 90% accuracy microarray. Functional clustering genes with greater than or less 1.75-fold change (86 genes) revealed RET/PTC3-induced regulation functions apoptosis (Ripk3, Tdga), cell–cell signaling (Cdh6, Fn1), cell cycle (Il24), immune and inflammation response (Cxcl10, Scya2, Il6, Gbp2, Oas1, Tap1, RT1Aw2, C2ta, Irf1, Lmp2, Psme2, Prkr), metabolism (Aldob, Ptges, Nd2, Gss, Gstt1), signal transduction (Socs3, Nf1, Jak2, Cpg21, Dusp6, Socs1, Stat1, Stat3, Cish) transcription (Nr4a1, Junb, Hfh1, Runx1, Foxe1). Genes coding for proteins intracellular pathways activated cytokines chemokines were strongly represented, indicating critical role modulation response.