C-reactive protein inhibits adiponectin gene expression and secretion in 3T3-L1 adipocytes

• DOI: 10.1677/joe-07-0133
• Publication Date: 2007-07-19T21:28:09Z
• Authors: Guoyue Yuan; Xia Chen; Qinyun Ma; Jie Qiao; Rongying Li; Xuesong Li; Shengxian Li; Jinfeng Tang; Libin Zhou; Huaidong Song; Mingdao Chen
• Keywords: 3T3-L1

C-reactive protein (CRP) is considered as one of the most sensitive markers inflammation. The aim present study to investigate effects CRP on production adiponectin in 3T3-L1 adipocytes. Northern and western blot analysis revealed that treatment inhibited mRNA expression secretion a dose- time-dependent manner. Co-incubation adipocytes with rosiglitazone decreased induction gene by rosiglitazone. However, luciferase reporter assays did not show affected activity approximately 2.1 kb promoter, which was increased alone. Pharmacological inhibition phosphatidylinositol (PI)-3 kinase LY294002 partially reversed CRP. These results collectively suggest suppresses through PI-3 pathway, might represent mechanism regulates insulin sensitivity.