Endoplasmic reticulum stress is involved in myocardial apoptosis of streptozocin-induced diabetic rats

• DOI: 10.1677/joe-07-0230
• Publication Date: 2008-02-29T18:52:07Z
• Authors: Zhenhua Li; Tao Zhang; Hongyan Dai; Guanghui Liu; Haibin Wang; Yingying Sun; Yun Zhang; Zhiming Ge
• Keywords: Streptozocin

Apoptosis plays a critical role in the diabetic cardiomyopathy, and endoplasmic reticulum stress (ERS) is one of intrinsic apoptosis pathways. Previous studies have shown that becomes swollen dilated myocardium, ERS involved heart failure kidney. This study aimed to demonstrate whether induced myocardium streptozotocin (STZ)-induced rats. We established type 1 rat model, used echocardiographic evaluation, hematoxylin–eosin staining, terminal deoxynucleotidyl transferase-mediated DNA nick-end labeling staining identify existence cardiomyopathy enhanced heart. performed immunohistochemistry, western blot, real-time PCR analyze hallmarks include glucose-regulated protein 78, CCAAT/enhancer-binding homologous (CHOP) caspase12. found these expression mRNA levels myocardium. Also, another pathway can lead cell death ERS, c-Jun NH 2 -terminal kinase-dependent pathway, was also activated Those results suggested STZ-induced rats' ERS-associated occurred pathophysiology cardiomyopathy.