Deceleration by angiotensin II of the differentiation and bone formation of rat calvarial osteoblastic cells

• DOI: 10.1677/joe.0.1560543
• Publication Date: 2004-12-02T23:42:13Z
• Authors: H Hagiwara; Y Hiruma; A Inoue; A Yamaguchi; S Hirose
• Keywords: Von Kossa stain

We examined the effects of angiotensin II (Ang II) on differentiation rat calvarial osteoblastic cells and formation bone by these cells. Northern blotting analysis revealed that Ang inhibited expression mRNA for osteocalcin, which is a protein specifically expressed during maturation decreased activity alkaline phosphatase, marker differentiation, in cells, acting via type 1 (AT1) receptor. used von Kossa staining to examine mineralized nodules Both number total area were quantified shown be 10(-7) M II. The accumulation calcium matrix layer was also Binding with subtype-specific antagonists presence AT1 receptors this culture system. caused marked increase rate production intracellular cAMP Our data suggest might intimately involved metabolism through its interaction