Liver receptor homologue-1 is expressed in human steroidogenic tissues and activates transcription of genes encoding steroidogenic enzymes

• DOI: 10.1677/joe.0.174r013
• Publication Date: 2004-12-02T23:42:13Z
• Authors: R Sirianni; JB Seely; G Attia; DM Stocco; BR Carr; V Pezzi; WE Rainey
• Keywords: Steroidogenic factor 1; Liver receptor homolog-1; Steroid 11-beta-hydroxylase

In the current study we test hypothesis that liver receptor homologue-1 (LRH; designated NR5A2) is involved in regulation of steroid hormone production. The potential role LRH was assessed by first examining expression human steroidogenic tissues and second effects on transcription genes encoding enzymes steroidogenesis. closely related to factor 1 (SF1; NR5A1), which expressed most regulates several steroid-metabolizing enzymes. transcripts were at high levels ovary testis. Adrenal placenta much lower than either or liver. To examine capacity used reporter constructs prepared with 5'-flanking region acute regulatory protein (StAR), cholesterol side-chain cleavage (CYP11A1), 3beta hydroxysteroid dehydrogenase type II (HSD3B2), 17alpha hydroxylase, 17,20 lyase (CYP17), 11beta hydroxylase (CYP11B1) aldosterone synthase (CYP11B2). Co-transfection these vector demonstrated like SF1, enhanced activity driven flanking DNA from StAR, CYP11A1, CYP17, HSD3B2, CYP11B1. Reporter CYP11A1 CYP17 increased co-transfection SF1. Of promoters examined only HSD3B2 more sensitive level ovarian testicular make it likely plays an important gonadal function.