Aqp2-Expressing Cells Give Rise to Renal Intercalated Cells

• DOI: 10.1681/asn.2012080866
• Publication Date: 2013-01-11T05:47:57Z
• Authors: Hongyu Wu; Lihe Chen; Qiaoling Zhou; Xi Zhang; Stefan Berger; Jiong Bi; Dorothy E. Lewis; Yang Xia; Wenzheng Zhang
• Keywords: Intercalated Cell; Aquaporin 2; Homeostasis

The mammalian collecting duct comprises principal and intercalated cells, which maintain sodium/water acid/base balance, respectively, but the epigenetic contributors to differentiation of these cell types remain unknown. Here, we investigated whether histone H3 K79 methyltransferase Dot1l, is highly expressed in participates this process. Taking advantage distribution aquaporin 2 (Aqp2), localizes cells duct, developed mice lacking Dot1l Aqp2-expressing (Dot1lAC) found that had approximately 20% fewer 13%–16% more than control mice. This deletion abolished methylation unexpectedly, most also undetectable di-methyl K79, suggesting Aqp2+ give rise cells. These cell-derived were present both developing mature kidneys. Furthermore, compared with mice, Dot1lAC 40% higher urine volume 18% lower osmolarity relatively normal electrolyte acid-base homeostasis. In conclusion, data suggest facilitates some α- β-intercalated from progenitor or