Induction of clusterin in tubules of nephrotic rats.

• DOI: 10.1681/asn.v9133
• Publication Date: 2021-04-26T19:22:24Z
• Authors: R Correa-Rotter; M E Ibarra-Rubio; G Schwochau; C Cruz; J R Silkensen; J Pedraza-Chaverri; D Chmielewski; M E Rosenberg
• Keywords: Clusterin; Renal cortex; Puromycin; Nephrosis

Clusterin is a glycoprotein induced after renal tubular cell injury. The purpose of this study was to examine the expression clusterin in disease model characterized early its course by predominant glomerular Male Wistar rats (weighing 251 +/- 16 g) were treated with puromycin aminonucleoside (PAN: 15 mg/100 g body wt, subcutaneously; n = 7) or vehicle (control; 8). kidneys harvested 6 d treatment, when nephrotic. mRNA markedly nephrotic (8.5-fold versus control). Immunohistochemistry studies demonstrated primarily tubules cortex and medulla. Many staining for dilated but had no other differentiating morphologic features. Increased numbers proliferating cells seen at d, there correlation between these staining. In contrast extent pattern staining, vimentin only sporadic, tubules, addition expected localization. An increase not 1, 2, 4 PAN injection. conclusion, epithelial induction occurs rats. appearance precedes development tubulointerstitial may be response proteinuria.