Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients

• DOI: 10.1684/ecn.2014.0348
• Publication Date: 2019-08-02T13:24:44Z
• Authors: Martin Kováč; Martina Vášková; Denisa Petráčková; Vendula Pelková; Ester Mejstříková; Tomáš Kalina; Markéta Žaliová; Jaroslav Weiser; Jan Starý; Ondřej Hrušák
• Keywords: Pathogenesis

Acute lymphoblastic leukemia (ALL) cells depend on the microenvironment of host in vivo and do not survive vitro culture. Conversely, suppression non-malignant tissues is one leading characteristics course ALL. Both malignant cell survival may be partly affected by soluble factors within bone marrow (BM) environment. Here, we aimed to identify proteins BM plasma children with ALL that contribute aggressiveness and/or microenvironment-mediated cells. LBMp (leukemic plasma) at time diagnosis was compared control (CBMp) or peripheral blood (CPBp) using a cytokine antibody array. The array enabled simultaneous detection 79 per sample. Candidate exhibiting significantly different profiles were further analyzed confirmed ELISA. mRNA expression candidate (TIMP1) studied quantitative reverse transcriptase polymerase chain reaction (qRTPCR). experiments identified 23 differed (p<0.05); these, two (TIMP1 LIF) withstood Bonferroni correction. In contrast, little difference observed between CBMp CPBp. At ALL, changes are detectable plasma. These probably participate pathogenesis result from composition.