The Effects of (-)-Epigallocatechin-3-Galate on Wound Closure and Infections in Mice

• DOI: 10.17140/sroj-1-101
• Publication Date: 2015-03-26T14:13:55Z
• Authors: Andrew R. Osterburg; Ryo Yamaguchi; ; Chad T. Robinson; ; S.H. Hyon; ; George F. Babcock
• Keywords: Myofibroblast

In this study, we investigated the effects of (-)-epigallocatechin-3-gallate (EGCG) on wound healing both in vitro and vivo with without infection.EGCG has antimicrobial properties could be useful as a topical agent to prevent and/or treat infections.Normal fibroblasts were isolated from dermis C57BL/6 mice cultured 0, 0.001 0.400 mg/ml EGCG.In assays demonstrated that migration, proliferation, apoptosis inhibited at EGCG concentrations 0.100-0.400mg/ml.Expression α-smooth muscle actin (α-SMA) was also reduced.In experiments measured closure/contraction full-thickness dorsal wounds treated 0.3, 3.0, 30.0 every 24 hours.Macrophages (F4/80), neutrophils (Ly-6G) myofibroblasts assessed 48 168 hours.By hours there significant reduction presence 30 dose vs. 0.3 3.0 (p<0.009,p<0.006, respectively).The percentage closure one week 87.87% (0.03 mg), 85.23% (0.3 mg) 40.06% (30 mg/ml) compared controls.Reduced quantities α-SMA observed 3 mg treatment group controls hours.We previously (MIC 50 ~0.3mg/ml).This data suggests potentially applied surface an negatively influencing healing.To end (10 model infected traumatic wound.EGCG significantly reduced bacterial load after two regimens.