Study of the cytotoxic effects of 2,5-diaziridinyl-3,6-dimethyl-1,4-benzoquinone (MeDZQ) in mouse hepatoma cells.

• DOI: 10.17179/excli2016-783
• Publication Date: 2017-01-01
• Authors: Rasa Jarašienė-Bu...; Milda Alksnė; Violeta Bartuskiene; Violeta Voišnienė; Jaroslav Burinskij; Narimantas Čėnas; Virginija Bukelsk...
• Keywords: Mode of Action; Benzoquinone

A number of quinones have been shown to be efficient anticancer agents. However, some mechanisms their action, in particular cell signaling are not well understood. The aim this study was partly fill gap by characterizing the mode cytotoxicity 2,5-diaziridinyl-3,6-dimethyl-1,4-benzoquinone (MeDZQ) malignant mouse hepatoma cells (MH-22A) with regard expression and activation main molecules MAPK pathway. revealed unequal roles MAP kinases MeDZQ-induced death: compound did induce significant changes ERK or its phosphorylation; JNK appeared responsible for survival, however, p38 kinase involved death. In order assess enzymatic action MeDZQ, we also found that antioxidant N,N'-diphenyl-p-phenylene diamine, iron-chelating agent desferrioxamine, DT-diaphorase inhibitor, dicoumarol, protected from MeDZQ cytotoxicity. It points parallel oxidative stress bioreductive alkylation modes MeDZQ.