anti inflammatory activity of hydrosols from tetragonia tetragonoides in lps induced raw 264 7 cells

• DOI: 10.17179/excli2017-121
• Publication Date: 2017-01-01
• Authors: Ko, Eun-Yi; Cho, Su-Hyeon; Kang, Kyungpil; Kim, Gibeom; Lee, Ji-Hyeok; Jeon, You-Jin; Kim, Daekyung; Ahn, Ginnae; Kim, Kil-Nam
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; hydrosols; MAPKs; 610; Original Article; Tetragonia tetragonoides; NF- κB; NF-κB; info:eu-repo/classification/ddc/610; anti-inflammatory

The present study was performed to investigate the anti-inflammatory activity of Tetragonia tetragonoides hydro- sols (TTH) and its underlying mechanism in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Gas chromatog- raphy (GC) coupled with mass spectrometry and retention index calculations showed that TTH were mainly com- posed of tetratetracontane (29.5 %), nonacosane (27.6 %), and oleamide (17.1 %). TTH significantly decreased the production of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin (IL)-6, and IL-1β in LPS-stimulated RAW 264.7 cells. Consistent with these observations, TTH treatment decreased the protein expression levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). The molecular mechanism of its anti-inflamma- tory activity was found to be associated with inhibition of nuclear factor-kappa B (NF-κB) phosphorylation and nuclear translocation of NF-κB 65. Furthermore, TTH markedly suppressed the LPS-induced phosphorylation of mitogen-activated protein kinases (MAPKs). Taken together, these data indicate that TTH exerts an anti-inflam- matory activity by inhibiting the NF-κB and MAPK signaling pathways in LPS-stimulated RAW 264.7 cells.<br/>EXCLI Journal;Vol. 16 2017<br/>