Tumor-derived urinary exosomal long non-coding RNAs as diagnostic biomarkers for bladder cancer.

• DOI: 10.17179/excli2019-1683
• Publication Date: 2020-01-01
• Authors: Maryam Abbastabar; Mohammad Sarfi; Abolfazl Golestani; Ali Karimi; Gholamreza Pourmand; Ehsan Khalili
• Keywords: Exosome; Diagnostic biomarker

Bladder cancer (BC) is the sixth most common malignancy in men and 17th women. Exosomal long non-coding RNAs (lncRNAs) have been defined as a novel biomarker for BC. The aim of this study to evaluate clinical significance urine exosomal PVT-1, ANRIL PCAT-1 BC patients with tumors classified T1 or T2. Exosomes were isolated from healthy donors, then characterized according their shape, size, exosome markers by Electron Microscopy, Dynamic light scattering, Western blotting. lncRNAs extraction was done determine expression levels qRT-PCR. significantly higher compared normal subjects. To performance identified detection, we performed ROC curves analysis. diagnostic accuracy PCAT-1, measured AUC, 0.7229 (sensitivity = 46.67 % specificity 87.5 %) 0.7292 43.33 %). Transcript urinary exosomes are potential biomarkers bladder cancer.