Immunomodulatory components of Trichinella spiralis excretory-secretory products with lactose-binding specificity.

• DOI: 10.17179/excli2022-4954
• Publication Date: 2022-01-01
• Authors: Nataša Ilić; Žanka Bojić‐Trboj...; Britta Lundström‐...; Danica Ćujić; Ivana Mitić; Alisa Gruden‐Movs...
• Keywords: Trichinella spiralis; Galectin

The immunomodulatory potential of Trichinella spiralis muscle larvae excretory-secretory products (ES L1) has been well documented in vitro on dendritic cells (DCs) and animal models autoimmune diseases. ES L1 possess the to induce tolerogenic DCs consequently trigger regulatory mechanisms that maintain immune homeostasis. use as a treatment for various inflammatory disorders proved be beneficial models, although precise factors have not yet identified. This study aimed at isolation characterization components galectin family member properties. Galectin-1-like proteins (TsGal-1-like) were isolated from based assumption existence lactose-specific carbohydrate-recognition domain recognized by anti-galectin-1 antibodies Western blot. TsGal-1-like isolate, similar galectin-1, induced with properties hence, capacity polarize T cell response towards type. was reflected significantly increased percentage CD4+CD25+Foxp3+ expression IL-10 TGF-β within this population. Proteomic analysis isolate mass spectrometry identified nineteen proteins, seven annotated function after blast against database T. UniProt database. To our surprise, none possesses homology known members. Nevertheless, certain galectin-1 properties, such specific lactose binding elicit response, so it would worth further investigating structure sugar its biological significance.