Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice

• DOI: 10.17305/bb.2023.9843
• Publication Date: 2023-10-30T07:28:23Z
• Authors: Nejc Umek; Luka Pušnik; Chiedozie Kenneth...; Žiga Šink; Simon Horvat; Jiří Janáček; Erika Cvetko
• Keywords: QH301-705.5; izoforme težke verige miozina; muscle fiber composition; sestava mišičnih vlaken; Streptozocin; capillary network analysis; Diabetes Mellitus, Experimental; Mice; Imaging, Three-Dimensional; 1 diabetes mellitus (T1DM); Type 1 diabetes mellitus (T1DM); 3D image analysis; sladkorna bolezen tipa 1 (T1DM); analiza 3D slike; Animals; analiza kapilarnih mrež; skeletal muscle; Biology (General); type 1 diabetes mellitus (T1DM); skeletna mišica; Muscle, Skeletal; sladkorna bolezen tipa 1 (T1DM), skeletna mišica, izoforme težke verige miozina, analiza kapilarnih mrež, sestava mišičnih vlaken, analiza 3D slike; Myosin Heavy Chains; three-dimensional (3D) image analysis; muscle fibre composition; Capillaries; myosin heavy chain isoforms; Mice, Inbred C57BL; Diabetes Mellitus, Type 1; info:eu-repo/classification/udc/611; Female; type 1 diabetes mellitus (T1DM), skeletal muscle, myosin heavy chain isoforms, capillary network analysis, muscle fibre composition, 3D image analysis; myosin heavy chain (MyHC) isoforms; Research Article

It is not well-understood how type 1 diabetes (T1DM) affects skeletal muscle histological phenotype, particularly capillarisation. This study aimed to analyze myosin heavy chain (MyHC) fibre changes and 3D capillary network characteristics in experimental T1DM mice. Female C57BL/6J-OlaHsd mice were categorized into streptozotocin (STZ)-induced diabetic (n = 12) age-matched non-diabetic controls =12). The phenotype of the soleus, gluteus maximus, gastrocnemius muscles characterized based on expression MyHC isoforms, while capillaries maximus assessed with immunofluorescence staining, confocal laser microscopy image analysis. STZ-induced exhibited elevated glucose levels, reduced body weight, prolonged thermal latency, verifying phenotype. In both mice, predominantly expressed fast-twitch 2b fibers, no significant differences noted. However, soleus had a greater proportion 2a fibers comparable fiber densities (26.2 ± 14.6% vs 21.9 13.5%) relative showed diameters (P 0.026), analysis indicated higher length per volume compared < 0.05). Overall, induced muscle, including shifts types, decreased diameters, increased capillarisation, possibly due atrophy. Our findings emphasize superior detail provided by analytical method for characterizing architecture, highlighting caution interpreting 2D data T1DM.