JAM3: A prognostic biomarker for bladder cancer via epithelial–mesenchymal transition regulation

• DOI: 10.17305/bb.2024.9979
• Publication Date: 2024-03-11T13:19:00Z
• Authors: Zhong-qi Pang; Jian-she Wang; Jin-feng Wang; Ya-xuan Wang; Bo Ji; Yi-dan Xu; Jia-xin He; Lu Zhang; Li-qiu Zhang; Bei-chen Ding; Yang Liu; Ming-hua Ren
• Keywords: Male; Gene Expression Regulation, Neoplastic; Epithelial-Mesenchymal Transition; Urinary Bladder Neoplasms; Cell Movement; Cell Line, Tumor; Biomarkers, Tumor; Humans; Female; Middle Aged; Prognosis; Cell Adhesion Molecules; Research Article

Understanding the intricate relationship between prognosis, immune function, and molecular markers in bladder cancer (BC) demands sophisticated analytical methods. To identify novel biomarkers for predicting prognosis function BC patients, we combined weighted gene co-expression network analysis (WGCNA) least absolute shrinkage selection operator (LASSO) regression analysis. This was conducted using data from The Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases. Ultimately, screened junctional adhesion molecule 3 (JAM3) as an independent risk factor BC. High levels of JAM3 were linked to adverse clinical parameters, such higher T N stages. Additionally, a JAM3-based nomogram model accurately predicted 1-, 3- 5-year survival rates indicating potential utility. Functional enrichment revealed that high expression activated calcium signaling pathway, extracellular matrix (ECM)-receptor interaction, PI3K-Akt positively correlated with genes associated epithelial–mesenchymal transition (EMT). Subsequently, found overexpression promoted migration invasion abilities cells, regulating N-cadherin, metallopeptidase 2 (MMP2), Claudin-1 thereby promoting EMT levels. showed negatively anti-tumor cells CD8+ while pro-tumor M2 macrophages, suggesting its involvement cell infiltration. checkpoint CD200 also positive correlation JAM3. Our findings elevated are predictive poor infiltration patients by process.