Affordable immunofluorescence staining on bones or other tissues without cryostat v1

• DOI: 10.17504/protocols.io.14egn2zpzg5d/v1
• Publication Date: 2022-10-17T17:27:23Z
• Authors: Vincent Fregona; Laetitia Largeaud
• Keywords: Immunofluorescence; Cryostat; Unicorn; Multiplex

We are happy to share the First UNICORN ★ Protocol on behalf of Hemato. How perform affordable immunofluorescence staining bones or other tissues without cryostat This method is an adaptation from - Multicolor quantitative confocal imaging cytometry published by Daniel Coutu et al. in Nature Method 2017 (see supplemental methods compose your buffers!) IBEX: A versatile multiplex optical approach for deep phenotyping and spatial analysis cells complex Andrea Radtke PNAS 2020 Not being experts microscopy, we realized that you microscopists MacGyvers bench