Serapure Beads Preparation and Testing v1

• DOI: 10.17504/protocols.io.3byl49952go5/v1
• Publication Date: 2024-06-01T04:08:33Z
• Authors: rute.carvalho Car...; Colleen Kellogg; Matt Lemay

This protocol is used to prepare low-cost SPRI beads for Illumina Library preparations. As part of the Hakai Institute Ocean Observing Program, from 0 m near bottom (260 m), biomolecular samples have been collected weekly genetically characterize plankton communities in Northern Salish Sea since 2015. These clean up PCR products 16S, 18S, COI, and 12S amplicons, implemented as a standard procedure eDNA analysis. modification following protocol: B. Faircloth T. Glenn. Serapure v2.2. Ecol. Evol. Biology, UCLA, November 19, 2011 Which was based on article: