Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids v1

• DOI: 10.17504/protocols.io.5jyl8jon8g2w/v1
• Publication Date: 2023-03-30T18:49:45Z

Fontana-Masson staining is a silver staining technique that is commonly used to identify melanin-containing cells. We combined it with DAB-TH staining that works by using an antibody to detect the presence of TH, followed by a reaction with a substrate (DAB) that results in the formation of a brown-colored product at the site of the antigen-antibody interaction.