MSD Gold Streptavidin Antibody Preparation and Plate Run Protocol v1

• DOI: 10.17504/protocols.io.5qpvobkj9l4o/v1
• Publication Date: 2022-05-16T20:55:50Z
• Authors: Kamaljot Gill; Maria Sckaff; Claire D Clelland

This protocol describes how to conjugate antibodies and run the Meso Scale Discovery (MSD) Sandwich enzyme-linked immunosorbent assay (ELISA) on MSD GOLD 96-well Small Spot Streptavidin SECTOR Plates. This protocol is adapted from MSD GOLD™ Streptavidin Plate and Avidin Plates Quick Guide 1 and MSD GOLD™ SULFO-TAG NHS-Ester Conjugation Quick Guide 2 and optimized for C9orf72 dipeptide repeat detection from human iPSC derived neurons.