Agrobacterium-mediated transformation of Diplodia sapinea v1

• DOI: 10.17504/protocols.io.5qpvok7ozl4o/v1
• Publication Date: 2024-08-02T09:29:56Z
• Authors: Anne Geertje Oost...; Laura Brodde; Miriam von Bargen; Bernard Slippers; Yvonne Becker; Ulrike Brandt; Frank Klawonn; Christiaan Grobler; Lucas Well; Jan Stenlid; Jonàs Oliva; Malin Elfstrand; André Fleißner
• Keywords: Heterologous

This protocol details an Agrobacterium-mediated genetic transformation method for the fungal plant pathogen Diplodia sapinea. The technique results in high rates of homologous integration, enabling both targeted mutagenesis and heterologous gene expression.