CHIP-reCHIP Protocol for Mapping Bivalent Chromatin v1

• DOI: 10.17504/protocols.io.8epv5x815g1b/v1
• Publication Date: 2024-02-22T15:31:10Z
• Authors: Janith A Seneviratne; William W H Ho; Eleanor Glancy; Melanie A Eckersl...
• Keywords: Bivalent (engine)

This is a detailed protocol for performing sequential ChIP-reChIP to map H3K4me3-H3K27me3 bivalent chromatin regions. It has been optimised using mouse embryonic stem cells and so may need be refined based on your cell type of interest. For more details please refer the accompanying manuscript (Hoet al. 2023).