High-quality RNA purification with on-column DNase treatment from tissue specimens v1

• DOI: 10.17504/protocols.io.8ufhwtn
• Publication Date: 2020-01-13T09:58:22Z
• Authors: Magda Bletsa; Antonios Fikatas; Sophie Gryseels; Jan Felix Drexler; Philippe Lemey; Yiqiao Li
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences

This protocol can be used for total RNA purification from tissue samples. It includes an intermediate on-column DNase treatment, which results in higher RNA yield and purity. This method is mainly optimised to obtain hepacivirus RNA extracts for whole-genome sequencing, but it can also be used for any viral RNA purification from samples with lower viral loads.