Marchantia agrobacterium transformation of sporelings in multi-well plates (plus materials info) v2

• DOI: 10.17504/protocols.io.92ah8ae
• Publication Date: 2019-12-03T18:31:26Z
• Authors: Linda Silvestri; Eftychis Frangedakis; Susana Sauret-Gueto; Marius Rebmann; Marta Tomaselli
• Keywords: Marchantia polymorpha

A modification of the Ishizaki et al 2008 Agrobacterium mediated Marchantia sporeling transformation protocol is used. Sterilised spores are grown for 5 days in 0.5x strength Gamborg plates prior to co-cultivation 2 with agrobacterium liquid media multiwell plates. Sporelings then spread on appropriate selective antibiotic. In about 7 days, positive transformants start emerge.