Study of MAIT Cell Activation in Viral Infections In Vivo v1

• DOI: 10.17504/protocols.io.bmg4k3yw
• Publication Date: 2021-09-22T11:39:46Z
• Authors: Timothy S C Hinks; Bonnie van Wilgen...; Huimeng Wang; Liyen Loh; Marios Koutsakos; Katherine Kedzierska; Alexandra J. Corbett; Zhenjun Chen
• Keywords: Adoptive Cell Transfer; Intracellular parasite

MAIT cells are abundant, highly evolutionarily conserved innate-like lymphocytes expressing a semi-invariant T cell receptor (TCR), which recognizes microbially derived small intermediate molecules from the riboflavin biosynthetic pathway. However, in addition to their TCR-mediated functions they can also be activated TCR-independent manner via cytokines including IL-12, -15, -18, and type I interferon. Emerging data suggest that expanded by range of viral infections, significantly contribute protective anti-viral response. Here we describe methods used investigate these vivo murine models. To overcome technical challenge rare specific pathogen-free laboratory mice, how pulmonary using intranasal bacterial infection or combination synthetic antigen TLR agonists. We protocols for adoptive transfer cells, lung homogenization plaque assays, surface intracellular cytokine staining determine activation.