V.3 - Direct wastewater RNA capture and purification via the "Sewage, Salt, Silica and SARS-CoV-2 (4S)" method v3

• DOI: 10.17504/protocols.io.bngsmbwe
• Publication Date: 2020-10-19T20:10:36Z
• Authors: Oscar N Whitney; Basem Al-Shayeb; Alex Crits-Cristoph; Mira Chaplin; Vinson Fan; Hannah Greenwald; Adrian Hinkle; Rose Kantor; Lauren Kennedy; Anna Maurer; Robert Tjian; Kara L. Nelson; UC Berkeley Waste...
• Keywords: 6. Clean water; 3. Good health

This protocol describes the procedure of the "4S" (Sewage, Salt, Silica and SARS-CoV-2) method for SARS-CoV-2 RNA extraction from wastewater. Offering a highly efficient, modular and economical alternative to existing wastewater RNA purification methods, this procedure lowers the barrier to entry for SARS-CoV-2 wastewater-based epidemiology. This procedure is intended to be carried out in a BSL2+ laboratory space, with precautions when handling raw wastewater samples.