Cytotoxicity Screening Assay - Paired with Antiviral Assays v1

• DOI: 10.17504/protocols.io.bp2l62mozgqe/v1
• Publication Date: 2025-03-18T21:51:12Z
• Authors: Briana L McGovern; Kris White

The following is a cytotoxicity protocol to be paired with a live virus antiviral screening in vitro. Cells are prophylactically treated with antiviral candidates that have been serially diluted to investigate activity, via CC50 values generated via analysis of MTT/MTS assays in uninfected cells. You may opt to include PgP inhibitor in the assay by adding 2uM into the infection media. For antiviral screenings for the family of virus you are working on, you will set up both an antiviral plate (infected) and a cytotoxicity plate (uninfected). The cytotoxicity plate will measure the cytotoxicity of the compounds on the cells.