SubARTIC ONT SARS-CoV-2 Spike sequencing protocol (LoCost) V3.2 v1

• DOI: 10.17504/protocols.io.btvnnn5e
• Publication Date: 2021-11-24T14:44:13Z
• Authors: Paul J Parsons; Gavin Horsburgh; Kathryn Maher; Steve Paterson; Terry Burke
• Keywords: Minion; Illumina dye sequencing; Amplicon; Amplicon sequencing

This protocol describes a procedure for sequencing the Spike gene of SARS-CoV-2 using short amplicons (146-208bp) with Oxford Nanopore technology (R9.4.1 MinION/GridION Flow Cell). The method has proved to be successful both clinical RNA samples and degraded wastewater samples. primers are unique SubARTIC method. library prep been heavily adapted from ncov-2019 v3 (ARTIC) by Josh Quick (https://www.protocols.io/view/ncov-2019-sequencing-protocol-v3-locost-bh42j8ye) "low cost" NEOF Liverpool Illumina ARTIC protocol. See this link version (https://www.protocols.io/view/sub-artic-illumina-sars-cov-2-spike-sequencing-pro-btpjnmkn).