Binding of Rab29 to the LRRK2 Armadillo domain by Microscale Thermophoresis v1

• DOI: 10.17504/protocols.io.bvamn2c6
• Publication Date: 2022-06-08T20:59:26Z
• Authors: Edmundo Vides
• Keywords: Microscale Thermophoresis; Microscale chemistry; Rab; Thermophoresis

Microscale thermophoresis (MST) is a powerful tool to measure the affinities of interactions between proteins. We present here our method for determining binding Rab29 GTPase LRRK2 (Leucine rich repeat kinase 2) N-terminal Armadillo domain. Work from several labs has shown that can recruit Golgi, where it normally resides, or other compartments, when artificially relocalized another cellular compartment. MST enabled us define precise site Rab GTPases on