Tandem affinity purification of a bait host protein in presence of a viral protein v1

• DOI: 10.17504/protocols.io.jeqcjdw
• Publication Date: 2017-08-27T12:47:05Z
• Authors: Benoit Besson; Florian Sonthonnax; Magalie Duchateau; Youcef Ben Khalifa; Florence Larrous; Hyeju Eun; Véronique Hourdel; Mariette Matondo; Julia Chamot-Rooke; Regis Grailhe; Hervé Bourhy
• Keywords: Tandem; Tandem affinity purification; Trap (plumbing); Protein purification

1st purification: a-FLAG (A2220; Sigma-Aldrich) 2nd GFP TRAP_A (ChromoTek)