Purification of His-Tagged Proteins using Ni-NTA Beads v2
DOI:
10.17504/protocols.io.kqdg3wz8ev25/v2
Publication Date:
2025-05-30T12:29:51Z
AUTHORS (4)
ABSTRACT
This protocol describes the batch purification of recombinant His-tagged proteins expressed in E. coli. Proteins containing a 6xHis tag are purified using Ni-NTA (Nickel-Nitrilotriacetic Acid) beads. The His-tag allows selective binding to nickel ions, enabling efficient purification under native conditions. After cell lysis, the lysate is incubated with Ni-NTA beads, followed by washing and elution using imidazole.
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