isolation of Salmonella and pathogenic E. coli in broiler farms in Uganda v1

• DOI: 10.17504/protocols.io.kxygxw17ov8j/v1
• Publication Date: 2025-01-15T13:48:45Z
• Authors: Thomas Ssemakadde; Nalumaga Pauline ...; Jude Collins Busi...; Joel Bazira; Kabanda Taseera
• Keywords: Isolation; Pathogenic Escherichia coli

This study investigated the prevalence, antimicrobial resistance, and extended-spectrum β-lactamase (ESBL) production in Escherichia coli Salmonella isolated from broiler farms Wakiso district, Uganda. A cross-sectional design was employed between August September 2021. total of 216 were selected using simple random sampling, pooled samples fresh fecal droppings cloacal swabs collected. Microbiological isolation identification conducted on selective media, followed by phenotypic genotypic assays to determine resistance profiles. Antibiotic susceptibility testing performed Kirby-Bauer disk diffusion method, results interpreted according CLSI 2020 guidelines. Phenotypic ESBL screened double-disc synergy method. DNA extraction conventional PCR confirm presence blaTEM gene. Sdata analysed Stata identify significant associations variables. The findings highlight a prevalence emergence ESBL-producing farms. calls for stewardship poultry production.