Automated BioID sample preparation v1

• DOI: 10.17504/protocols.io.kxygxymdwl8j/v1
• Publication Date: 2024-04-11T15:11:20Z
• Authors: Emilio Cirri; Hannah Knaudt; Domenico Di Fraia; Nadine Pömpner; Norman Rahnis; Ivonne Heinze; Alessandro Ori; Therese Dau

We introduce an automated workflow for proximity dependent biotinylation on a liquid handler to process up to 96 samples at a time,combined with shorter liquid chromatography gradients and data-independent acquisition to increase analysis throughput and enable reproducible protein quantitation