Imaging of cholinergic interneurons in post-mortem rodent tissue to identify striatal satellite astrocytes v1

• DOI: 10.17504/protocols.io.kxygxypbzl8j/v1
• Publication Date: 2024-08-23T00:26:00Z
• Authors: Shinil Raina; Stephanie J Cragg

This protocol details confocal image acquisition steps and the analysis pipeline used to calculate inter-soma distance between cholinergic interneurons/pMSNs and their nearest astrocyte. The partner Protocol: Immunofluorescent Labelling of Post-Mortem Rodent Brain Tissue describes how to label cholinergic interneurons/pMSNs along with striatal astrocytes in PFA perfusion-fixed, 50-µm thick, post-mortem rodent brain tissue.