Cryo-electron tomography of thinned synapses v1

• DOI: 10.17504/protocols.io.n92ldm188l5b/v1
• Publication Date: 2024-07-10T14:09:25Z
• Authors: Anna Siegert; Arsen Petrovic; Thanh Thao Do; Florelle Domart; Rubén Fernández-B...
• Keywords: Cryo-electron tomography

Here we provide a protocol for cryo-electron tomography (cryo-ET) of thinned synapses within intact rat primary neuron cultures. This workflow relies on cryo-focused-ion-beam (FIB) milling to enable unrestricted access neuronal cultures and achieve samples sufficiently thin (~150 nm) high resolution cryo-ET imaging. allows targeting with without cryo-fluorescence light microscopy (cryo-FLM) correlation FIB cryo-ET.