Library preparation (dsDNA single indexing, non-UDG, no split) v1

• DOI: 10.17504/protocols.io.n92ldpjexl5b/v1
• Publication Date: 2023-05-08T20:31:21Z
• Authors: Marcel Keller; Christiana L Scheib

Protocol for the preparation of single indexed double-stranded DNA libraries Illumina sequencing, optimized ultra-short ancient molecules, modified from Meyer & Kircher (2010) Cold Spring Harb. Protoc. (doi: 10.1101/pdb.prot5448). This protocol does not include treatment with UDG (USER) to remove damage in form deaminated cytosines.