Use of the waxworm Galleria mellonella larvae as an infection model to study Acinetobacter baumannii v2

• DOI: 10.17504/protocols.io.n92ldpr38l5b/v2
• Publication Date: 2023-12-22T19:47:55Z
• Authors: Kah Ern Ten; Nazmul Hasan Muzahid; Sadequr Rahman; tan.hocksiew not ...
• Keywords: Galleria mellonella; Acinetobacter baumannii; Human pathogen

Galleria mellonella larvae have been increasingly used in various scientific research, including microbial infection studies. They act as suitable preliminary models to study host-pathogen interactions due their advantages, such the ability survive at 37 °C mimicking human body temperature, immune system shares similarities with mammalians, etc. Here, we presented a protocol for simple rearing and maintenance of science research laboratories without requiring special instruments specialised training. This allows continuous supply healthy purposes. Besides, this also provides detailed procedures on (i) assays (killing assay bacterial burden assay) virulence studies (ii) cell harvesting from infected RNA extraction gene expression during infection. Our could not only be A. baumannii but can modified according different strains. The last step contains supplemental video extra context tips, part protocols.io Spotlight series, featuring conversations authors.